All lanes : Anti-Serum Amyloid P antibody (ab66715) at 1 µg/mlLane 1 : Bladder (Human) Whole Cell Lysate - adult normal tissue (ab29491)Lane 2 : Human Platelet (Human adult normal cell line) Whole Cell Lysate Lane 3 : Human Plasma Total Protein Lysate Lane 4 : Bone Marrow (Human) Tissue Lysate - adult normal tissue Lane 5 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 6 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 7 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.