All lanes : Anti-Seryl-tRNA synthetase antibody (ab50146) at 1/1000 dilutionLane 1 : Whole cell lysate from rat hippocampal neurons (untreated)Lane 2 : Whole cell lysate from rat hippocampal neurons (treated with neurotrophin)Lane 3 : Whole cell lysate from rat hippocampal neurons (treated with inhibitor of the neurotrophin pathway 1 + neurotrophin)Lane 4 : Whole cell lysate from rat hippocampal neurons (treated with inhibitor of the neurotrophin pathway 2 + neurotrophin)Lane 5 : Whole cell lysate from rat hippocampal neurons (treated with inhibitor of the neurotrophin pathway 1)Lane 6 : Whole cell lysate from rat hippocampal neurons (treated with inhibitor of the neurotrophin pathway 2)Lysates/proteins at 40 µg per lane.SecondaryAlkaline Phosphatase-conjugeted goat anti-rabbit IgG at 1/20000 dilutionPerformed under reducing conditions.
Anti-Seryl-tRNA synthetase antibody (ab50146) at 1/10000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/mlSecondaryGoat anti-rabbit IgG (H+L) at 1/10000 dilution