Anti-SESN1 antibody
| Name | Anti-SESN1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab67156 |
| Prices | $384.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Human |
| Antigen | Full length protein, corresponding to amino acids 1-551 of Human SESN1 |
| Description | Mouse Polyclonal |
| Gene | SESN1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-SESN1 antibody (ab67156) at 1/500 dilutionLane 1 : transfected 293T cell lysate Lane 2 : non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
Immunofluorescence of ab67156 at 10 µg/ml on HeLa cells
IHC image of ab67156 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab67156, at 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.