ab139987 staining SETD5 in human pancreatic cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with TBS + 0.05% Triton and blocked with 5% BSA for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in TBS) for 8 hours at 4°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.See Abreview
ab139987 staining SETD5 in mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with TBS + 0.05% Triton and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in TBS) for 8 hours at 4°C. A Biotin-conjugated goat anti-rabbit IgG monoclonal (1/1000) was used as the secondary antibody.See Abreview
Anti-SETD5 antibody (ab139987) at 1 µg/ml + Mouse stomach lysates at 10 µg