Anti-SFPQ antibody

• Supplier: Abcam
• Catalog: ab38148
• Prices: $390.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF IHC-P
• Species Reactivities: Mouse, Human, Rat, Zebrafish
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human SFPQ
• Description: Rabbit Polyclonal
• Gene: SFPQ
• Conjugate: Unconjugated

Product images

All lanes : Anti-SFPQ antibody (ab38148) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

ICC/IF image of ab38148 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab38148, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Anti-SFPQ antibody (ab38148) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

IHC image of SFPQ staining in Human colon cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38148, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunofluorescence analysis of mouse amygdala tissue, staining SFPQ (green) with ab38148 at 1/200 dilution. An AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.

Product References

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Innes BT, Bailey ML, Brandl CJ, Shilton BH, Litchfield DW. Front Physiol. 2013 Feb 13;4:18.

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O'Bryan MK, Clark BJ, McLaughlin EA, D'Sylva RJ, O'Donnell L, Wilce JA, Sutherland J, O'Connor AE, Whittle B, Goodnow CC, Ormandy CJ, Jamsai D. PLoS Genet. 2013;9(7):e1003628.

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Ke YD, Dramiga J, Schutz U, Kril JJ, Ittner LM, Schroder H, Gotz J. PLoS One. 2012;7(4):e35678.

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Seyfried NT, Gozal YM, Donovan LE, Herskowitz JH, Dammer EB, Xia Q, Ku L, Chang J, Duong DM, Rees HD, Cooper DS, Glass JD, Gearing M, Tansey MG, Lah JJ, Feng Y, Levey AI, Peng J. J Proteome Res. 2012 May 4;11(5):2721-38.

Quantitative profiling of in vivo-assembled RNA-protein complexes using a novel - Quantitative profiling of in vivo-assembled RNA-protein complexes using a novel

Tsai BP, Wang X, Huang L, Waterman ML. Mol Cell Proteomics. 2011 Apr;10(4):M110.007385.

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Guillaumond F, Boyer B, Becquet D, Guillen S, Kuhn L, Garin J, Belghazi M, Bosler O, Franc JL, Francois-Bellan AM. FASEB J. 2011 Aug;25(8):2740-56.

Proteomics analysis of the estrogen receptor alpha receptosome. - Proteomics analysis of the estrogen receptor alpha receptosome.

Nalvarte I, Schwend T, Gustafsson JA. Mol Cell Proteomics. 2010 Jul;9(7):1411-22.