Anti-SFPQ antibody
| Name | Anti-SFPQ antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab76955 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | FC WB ELISA |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Recombinant fragment: EEKISDSEGF KANLSLLRRP GEKTYTQRCR LFVGNLPADI TEDEFKRLFA KYGEPGEVFI NKGKGFGFIK LESRALAEIA KAELDDTPMR GRQ, corresponding to amino acids 269-362 of Human SFPQ (NP_005057) with tag |
| Description | Mouse Monoclonal |
| Gene | SFPQ |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-SFPQ antibody (ab76955) at 1 µg/ml + Recombinant tagged human SFPQ fragment at 0.2 µgSecondaryGoat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
Anti-SFPQ antibody (ab76955) at 1 µg/ml + HeLa nuclear lysate at 25 µgSecondaryGoat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
![Overlay histogram showing HeLa cells stained with ab76955 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76955, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/29085_SFPQ-Primary-antibodies-ab76955-1.jpg)
Overlay histogram showing HeLa cells stained with ab76955 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76955, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.