ab73891 staining SFRS3 in mouse E9.5 embryo tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% BSA + 10% goat serum + 0.1% Triton X-100 in PBS for 1 hour at 20°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA + 10% goat serum + 0.1% Triton) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.See Abreview
All lanes : Anti-SFRS3 antibody (ab73891) at 1/500 dilutionLane 1 : extracts from HeLa cellsLane 2 : extracts from Jurkat cellsLane 3 : extracts from Jurkat cells with immunizing peptide at 10 µgLysates/proteins at 30 µg per lane.
Left panel: immunofluorescence analysis of MCF7 cells, using 1/500-1/1000 ab73891; Right panel: same, after pre-incubation with the immunizing peptide.