Anti-SGLT1 antibody
| Name | Anti-SGLT1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab14685 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB DB ELISA |
| Species Reactivities | Mouse, Rat, Sheep, Human |
| Antigen | Synthetic peptide, corresponding to amino acids 603-623 of Human SGLT1 |
| Description | Rabbit Polyclonal |
| Gene | SLC5A1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab14685 (2µg/ml) staining SGLT1 in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the apical epithelium cells of the villi.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
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