Anti-SH3 containing Grb 2 like 1 protein antibody [2F5]

• Supplier: Abcam
• Catalog: ab114012
• Prices: $370.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 2F5
• Applications: IP WB IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Recombinant full length Human SH3 containing Grb 2 like 1 protein (NP_003016) produced in HEK293T cells
• Description: Mouse Monoclonal
• Gene: SH3GL1
• Conjugate: Unconjugated

Product images

All lanes : Anti-SH3 containing Grb 2 like 1 protein antibody [2F5] (ab114012) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY SH3 containing Grb 2 like 1 cDNALysates/proteins at 5 µg per lane.

All lanes : Anti-SH3 containing Grb 2 like 1 protein antibody [2F5] (ab114012) at 1/2000 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : HT29 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.

ab114012 at 1/100 dilution staining SH3 containing Grb 2 like 1 in COS7 cells transiently transfected by pCMV6-ENTRY SH3 containing Grb 2 like 1 and analyzed by immunofluorescence.

ab114012 at 1/150 dilution staining SH3 containing Grb 2 like 1 in Human bladder carcinoma tissue by immunohistochemistry (FFPE).

ab114012 staining SH3 containing Grb 2 like 1 in HeLa cells (red) compared to a non-specific negative control (blue), and analyzed by flow cytometry.

ab114012 staining SH3 containing Grb 2 like 1 in Jurkat cells (red) compared to a non-specific negative control (blue), and analyzed by flow cytometry.

ab114012 immunoprecipitating SH3 containing Grb 2 like 1 protein in human HEK293 cells transfected with human pCMV6-SH3GL1 whole cell lysate. 1000µg of cell lysate was incubated with primary antibody (1µl/mg in 1% NP40) and matrix (Protein G) for 2 hours at 4°C. For western blotting ab114012 (1/2000) was used to confirm successful immunoprecipation.See Abreview

Product References

Identification of SH3 domain proteins interacting with the cytoplasmic tail of - Identification of SH3 domain proteins interacting with the cytoplasmic tail of

Ebsen H, Lettau M, Kabelitz D, Janssen O. PLoS One. 2014 Jul 18;9(7):e102899.