Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane, cytoplasm and cell junctions. Recommended concentration of ab122310 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling SHCBP1L with ab122310. Staining shows strong cytoplasmic positivity in cells in seminiferous ducts.
All lanes : Anti-SHCBP1L antibody (ab122310) at 1/250 dilutionLane 1 : RT-4 cell lysateLane 2 : U-251 MG cell lysateLane 3 : Human plasmaLane 4 : Human Liver lysateLane 5 : Human Tonsil lysatedeveloped using the ECL technique