Anti-Shugoshin antibody
| Name | Anti-Shugoshin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58023 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-293 of Human Shugoshin |
| Description | Mouse Monoclonal |
| Gene | SGOL1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Western blot against tagged recombinant protein immunogen using ab58023 Shugoshin antibody at 1ug/ml.
ab58023 (1/200) detecting Shugoshin in assynchronous HeLa cells (green - image depicts a metaphase and an interphase cell). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI (red). Please refer to abreview for further experimental details.See Abreview
![Overlay histogram showing MCF-7 cells stained with ab58023 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58023, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/29893_Shugoshin-Primary-antibodies-ab58023-3.jpg)
Overlay histogram showing MCF-7 cells stained with ab58023 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58023, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
TRF1 ensures the centromeric function of Aurora-B and proper chromosome - TRF1 ensures the centromeric function of Aurora-B and proper chromosome
Ohishi T, Muramatsu Y, Yoshida H, Seimiya H. Mol Cell Biol. 2014 Jul;34(13):2464-78.
Mutations in SGOL1 cause a novel cohesinopathy affecting heart and gut rhythm. - Mutations in SGOL1 cause a novel cohesinopathy affecting heart and gut rhythm.
Chetaille P, Preuss C, Burkhard S, Cote JM, Houde C, Castilloux J, Piche J, Gosset N, Leclerc S, Wunnemann F, Thibeault M, Gagnon C, Galli A, Tuck E, Hickson GR, El Amine N, Boufaied I, Lemyre E, de Santa Barbara P, Faure S, Jonzon A, Cameron M, Dietz HC, Gallo-McFarlane E, Benson DW, Moreau C, Labuda D, Zhan SH, Shen Y, Jomphe M, Jones SJ, Bakkers J, Andelfinger G. Nat Genet. 2014 Nov;46(11):1245-9.
SGOL1 variant B induces abnormal mitosis and resistance to taxane in non-small - SGOL1 variant B induces abnormal mitosis and resistance to taxane in non-small
Matsuura S, Kahyo T, Shinmura K, Iwaizumi M, Yamada H, Funai K, Kobayashi J, Tanahashi M, Niwa H, Ogawa H, Takahashi T, Inui N, Suda T, Chida K, Watanabe Y, Sugimura H. Sci Rep. 2013 Oct 22;3:3012.
Bod1 regulates protein phosphatase 2A at mitotic kinetochores. - Bod1 regulates protein phosphatase 2A at mitotic kinetochores.
Porter IM, Schleicher K, Porter M, Swedlow JR. Nat Commun. 2013;4:2677.