Anti-Siah2 antibody
| Name | Anti-Siah2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab72064 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF ELISA WB |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Synthetic peptide derived from an internal sequence of human Siah2 |
| Description | Rabbit Polyclonal |
| Gene | SH3GL3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Siah2 antibody (ab72064) at 1/500 dilutionLane 1 : Extracts from COLO cellsLane 2 : Extracts from COLO cells with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.
ICC/IF image of ab72064 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72064, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.