Anti-SKI antibody
| Name | Anti-SKI antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab19864 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Human, Rat, Horse, Chicken, Dog, Xenopus |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 600 - 700 of Human c-Ski |
| Description | Rabbit Polyclonal |
| Gene | SKI |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-SKI antibody (ab19864) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ICC/IF image of ab19864 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19864, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Modulation of K-Ras-dependent lung tumorigenesis by MicroRNA-21. - Modulation of K-Ras-dependent lung tumorigenesis by MicroRNA-21.
Hatley ME, Patrick DM, Garcia MR, Richardson JA, Bassel-Duby R, van Rooij E, Olson EN. Cancer Cell. 2010 Sep 14;18(3):282-93.
Differential expression of SKI oncogene protein in hemangiomas. - Differential expression of SKI oncogene protein in hemangiomas.
O TM, Tan M, Tarango M, Fink L, Mihm M, Ma Y, Waner M. Otolaryngol Head Neck Surg. 2009 Aug;141(2):213-8. doi: