Anti-Skp1 antibody
| Name | Anti-Skp1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab10546 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Guinea Pig, Xenopus, Zebrafish |
| Antigen | Synthetic peptide derived from residues 50 - 150 of Human Skp1 |
| Description | Rabbit Polyclonal |
| Gene | SKP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab10546 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10546, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
developed using the ECL techniquePerformed under reducing conditions.
ab10546 was used in immunohistochemistry with paraffin embedded sections of human tonsil, using DAB as a chromogen (brown). Counterstaining of nuclei was performed with haemotoxylin (blue).Strong staining of epithelial cell nuclei is seen (and some staining of the cytoplasm) in this image.
developed using the ECL techniquePerformed under reducing conditions.
Product References
Rapid nutritional remodeling of the host cell upon attachment of Legionella - Rapid nutritional remodeling of the host cell upon attachment of Legionella
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Melanoma antigen gene protein-A11 (MAGE-11) F-box links the androgen receptor - Melanoma antigen gene protein-A11 (MAGE-11) F-box links the androgen receptor
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Proteomics analysis of Ring1B/Rnf2 interactors identifies a novel complex with - Proteomics analysis of Ring1B/Rnf2 interactors identifies a novel complex with
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The Arabidopsis F-box protein TIR1 is an auxin receptor. - The Arabidopsis F-box protein TIR1 is an auxin receptor.
Kepinski S, Leyser O. Nature. 2005 May 26;435(7041):446-51.