Overlay histogram showing HeLa cells stained with ab76502 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76502, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-Skp1 antibody [EPR3304] (ab76502) at 1/2000 dilutionLane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : PC-12 cell lysateLane 4 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP labeled IgG at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/487_ab76502.JPG)
All lanes : Anti-Skp1 antibody [EPR3304] (ab76502) at 1/2000 dilutionLane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : PC-12 cell lysateLane 4 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP labeled IgG at 1/2000 dilution
ab76502 staining Skp1 in HeLa cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in methanol, blocked with 1% BSA for 30 minutes at 24°C then incubated with ab76502 at a 1/500 dilution for 1 hour at 24°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.See Abreview