Anti-SLC25A12 antibody [EPR16294]

• Supplier: Abcam
• Catalog: ab200201
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16294
• Applications: IP WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SLC25A12 aa 600 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: SLC25A12
• Conjugate: Unconjugated

Product images

All lanes : Anti-SLC25A12 antibody [EPR16294] (ab200201) at 1/5000 dilutionLane 1 : Human fetal heart lysateLane 2 : Human skeletal muscle lysateLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-SLC25A12 antibody [EPR16294] (ab200201) at 1/1000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 2 : A431 (Human epidermoid carcinoma) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-SLC25A12 antibody [EPR16294] (ab200201) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse heart lysateLane 3 : Mouse kidney lysateLane 4 : Rat brain lysateLane 5 : Rat heart lysateLane 6 : Rat kidney lysateLane 7 : C6 (Rat glial tumor cells) whole cell lysateLane 8 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 9 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysateLane 10 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

SLC25A12 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200201 at 1/40 dilution.Western blot was performed from the immunoprecipitate using ab200201 at 1/1000 dilution.Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Jurkat whole cell lysate 10 µg (Input).Lane 2: ab200201 IP in Jurkat whole cell lysate.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200201 in Jurkat whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 30 second.

SLC25A12 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200201 at 1/40 dilution.Western blot was performed from the immunoprecipitate using ab200201 at 1/1000 dilution (Panel A) or ab192244 at 1/1000 dilution (Panel B).Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Jurkat whole cell lysate 10 µg (Input).Lane 2: ab200201 IP in Jurkat whole cell lysate.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200201 in Jurkat whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time (Panel A and Panel B): 30 second.