Anti-SLC7A5 antibody
| Name | Anti-SLC7A5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab85226 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P ELISA |
| Species Reactivities | Human |
| Antigen | A 16 amino acid synthetic peptide from near the N terminal residues of Human SLC7A5 protein (Swiss-Prot Q01650) |
| Blocking Peptide | SLC7A5 peptide |
| Description | Rabbit Polyclonal |
| Gene | SLC7A5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-SLC7A5 antibody (ab85226) at 1/200 dilution + human lung lysate
ab85226, at 1/100 dilution, staining SLC7A5 in formalin-fixed, paraffin-embedded human lung by Immunohistochemistry.
ICC/IF image of ab85226 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85226, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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Reidy PT, Walker DK, Dickinson JM, Gundermann DM, Drummond MJ, Timmerman KL, Cope MB, Mukherjea R, Jennings K, Volpi E, Rasmussen BB. J Appl Physiol (1985). 2014 Jun 1;116(11):1353-64. doi:
Bed rest impairs skeletal muscle amino acid transporter expression, mTORC1 - Bed rest impairs skeletal muscle amino acid transporter expression, mTORC1
Drummond MJ, Dickinson JM, Fry CS, Walker DK, Gundermann DM, Reidy PT, Timmerman KL, Markofski MM, Paddon-Jones D, Rasmussen BB, Volpi E. Am J Physiol Endocrinol Metab. 2012 May 15;302(9):E1113-22. doi:
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Drummond MJ, Glynn EL, Fry CS, Timmerman KL, Volpi E, Rasmussen BB. Am J Physiol Endocrinol Metab. 2010 May;298(5):E1011-8. doi: