Anti-Smac / Diablo antibody
| Name | Anti-Smac / Diablo antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab8114 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Peptide ( EERAESEQEAYLRED )corresponding to amino acids 225 to 239 of humanSmac/DIABLO (1) |
| Description | Rabbit Polyclonal |
| Gene | DIABLO |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Smac / Diablo antibody (ab8114) at 1 µg/mlLane 1 : human heart tissue lysate with absence of blocking peptideLane 2 : human heart tissue lysate with Smac / Diablo peptide (ab8380)
ab8114 at 5µg/ml staining Smac/Diablo in human ovary tissue by IHC
ICC/IF image of ab8114 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8114, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.