Anti-Smac / Diablo antibody [SMAC 17 1-87]

• Supplier: Abcam
• Catalog: ab111893
• Prices: $384.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: SMAC 17 1-87
• Applications: IP ICC/IF ICC/IF WB
• Species Reactivities: Rat, Human
• Antigen: Recombinant full length Human Smac/ Diablo with a His tag at C terminal end
• Description: Mouse Monoclonal
• Gene: DIABLO
• Conjugate: Unconjugated

Product images

ab111893 labelling Smac / Diablo (green) in the cytoplasm of A431 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse IgG was used as the secondary antibody. Red (phalloidiin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

ab111893 labelling Smac / Diablo (green) in the cytoplasm of MCF-7 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse IgG was used as the secondary antibody. Red (phalloidiin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

ab111893 labelling Smac / Diablo (green) in the cytoplasm of PC12 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse IgG was used as the secondary antibody. Red (phalloidiin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

All lanes : Anti-Smac / Diablo antibody [SMAC 17 1-87] (ab111893) at 1/1000 dilutionLane 1 : 293 cell lysateLane 2 : HeLa cell lysateLane 3 : PC12 cell lysateLysates/proteins at 25 µg per lane.