Anti-Smad4 antibody [SMD46 (same as DCS-46)] - ChIP Grade
| Name | Anti-Smad4 antibody [SMD46 (same as DCS-46)] - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab3219 |
| Prices | $395.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | SMD46 (same as DCS-46) |
| Applications | FC ICC/IF ICC/IF IP WB ChIP |
| Species Reactivities | Rabbit, Human |
| Antigen | Purified recombinant DPC4 protein |
| Description | Mouse Monoclonal |
| Gene | SMAD4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
The image is of a colony of undifferentiated human embryonic stem cells (H9). The cells are positive for Smad4 with staining localised in the nucleus. Dilution: 1/50
![Overlay histogram showing HeLa cells stained with ab3219 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3219, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/1950_Smad4-Primary-antibodies-ab3219-1.jpg)
Overlay histogram showing HeLa cells stained with ab3219 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3219, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
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Promoter-wide analysis of Smad4 binding sites in human epithelial cells. - Promoter-wide analysis of Smad4 binding sites in human epithelial cells.
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Dissecting signaling pathways that govern self-renewal of rabbit embryonic stem - Dissecting signaling pathways that govern self-renewal of rabbit embryonic stem
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p38 MAPK mediates fibrogenic signal through Smad3 phosphorylation in rat - p38 MAPK mediates fibrogenic signal through Smad3 phosphorylation in rat
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