All lanes : Anti-SMAP antibody (ab202282) at 0.1 µg/mlLane 1 : Whole cell lysate from HeLa cellsLane 2 : Whole cell lysate from 293T cellsLane 3 : Whole cell lysate from Jurkat cellsLane 4 : Whole cell lysate from mouse TCMK-1 cellsLane 5 : Whole cell lysate from mouse NIH 3T3 cellsLysates/proteins at 50 µg per lane.developed using the ECL techniqueExposure time : 3 minutes
Lane 1: Detection of Human SMAP by Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using ab202282 at 6 µg per reaction for IP.Lane 2: Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using an antibody which recognized an epitope of SMAP.Lane 3: Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using Control IgG.Subsequent Western blot detection used ab202282 at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30seconds.