All lanes : Anti-SMARCC1 antibody (ab22355) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 ug Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 ug with Human SMARCC1 peptide (ab24351) at 1 µg/mlSecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
ICC/IF image of ab22355 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab22355, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Image courtesy of Human Protein Atlasab22355 staining SMARCC1in human kidney. Paraffin embedded human kidney tissue was incubated with ab22355 (1/2000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab22355 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org