Anti-SMARCC1 antibody
| Name | Anti-SMARCC1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab72502 |
| Prices | $384.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP |
| Species Reactivities | Human, Rabbit, Horse, Guinea Pig, Bovine, Dog, Pig, Chimpanzee, Ferret, Monkey, Ape, Orangutan, Elephant |
| Antigen | Synthetic peptide corresponding to a region between residues 750 and 800 of human SMARCC1 (NP_003065 |
| Description | Rabbit Polyclonal |
| Gene | SMARCC1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab72502 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab72502 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol fixed (100%, 5min) HepG2 cells at 5ug/ml.
All lanes : Anti-SMARCC1 antibody (ab72502) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µg
Detection of SMARCC1 by Western Blot of Immunprecipitate. ab72502 at 1µg/ml staining SMARCC1 in HeLa whole cell lysate immunoprecipitated using ab72502 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.