All lanes : Anti-Smoothelin antibody (ab74379) at 1 µg/mlLane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : SK N SH (Human neuroblastoma) Whole Cell LysateLane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.