Anti-SMYD1 antibody
| Name | Anti-SMYD1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab34472 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human, Mouse, Chicken, Bovine, Zebrafish |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human SMYD1 |
| Blocking Peptide | Human SMYD1 peptide |
| Description | Rabbit Polyclonal |
| Gene | SMYD1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab34472 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34472, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
The myosin-interacting protein SMYD1 is essential for sarcomere organization. - The myosin-interacting protein SMYD1 is essential for sarcomere organization.
Just S, Meder B, Berger IM, Etard C, Trano N, Patzel E, Hassel D, Marquart S, Dahme T, Vogel B, Fishman MC, Katus HA, Strahle U, Rottbauer W. J Cell Sci. 2011 Sep 15;124(Pt 18):3127-36.
Crystal structure of cardiac-specific histone methyltransferase SmyD1 reveals - Crystal structure of cardiac-specific histone methyltransferase SmyD1 reveals
Sirinupong N, Brunzelle J, Ye J, Pirzada A, Nico L, Yang Z. J Biol Chem. 2010 Dec 24;285(52):40635-44.