All lanes : Anti-SNAP-beta antibody [EPR15046(B)] - N-terminal (ab184563) at 1/5000 dilutionLane 1 : A549 lysateLane 2 : HepG2 lysateLane 3 : SH SY5Y lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilutiondeveloped using the ECL technique
Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate immunoprecipitated using ab184563 at 1/50 dilution.Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Anti-SNAP-beta antibody [EPR15046(B)] - N-terminal (ab184563) at 1/5000 dilution + Human fetal brain lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilutiondeveloped using the ECL technique