Anti-SNAP23 antibody

• Supplier: Abcam
• Catalog: ab4114
• Prices: $398.00
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IHC-P ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Fusion protein corresponding to Human SNAP23
• Description: Rabbit Polyclonal
• Gene: SNAP23
• Conjugate: Unconjugated

Product images

Taken from Galli et al. 1998.

IHC image of ab4114 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4114, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

ab4114 staining SNAP23 in HT-1080 cells by Immunocytochemistry/ Immunofluorescence.Cells were grown on glass coverslips, treated with PMA and then fixed with 4% (w/v) paraformaldehyde in phosphate buffered saline (PBS). Samples were then permeabilized with 0.1% Triton X-100 in PBS and blocked with 5% (w/v) skimmed milk powder in PBS before staining with ab4114 and the appropriate secondary antibody, followed by washing and mounting.Scale bars: 10 µm.

Immunohistochemical analysis of rat pancreatic lobules, staining SNAP23 with ab4114. Following fixation in paraformaldehyde, cells were blocked for 30 min at RT, and primary antibody (1/50) was added simultaneously for 1.5 hours at RT. An AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.

Product References

The human submandibular gland: immunohistochemical analysis of SNAREs and - The human submandibular gland: immunohistochemical analysis of SNAREs and

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VAMP3, syntaxin-13 and SNAP23 are involved in secretion of matrix - VAMP3, syntaxin-13 and SNAP23 are involved in secretion of matrix

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Weng N, Thomas DD, Groblewski GE. J Biol Chem. 2007 Mar 30;282(13):9635-45. Epub 2007 Feb 1.

SNAP-23 and syntaxin-2 localize to the extracellular surface of the platelet - SNAP-23 and syntaxin-2 localize to the extracellular surface of the platelet

Flaumenhaft R, Rozenvayn N, Feng D, Dvorak AM. Blood. 2007 Sep 1;110(5):1492-501. Epub 2007 May 7.

Domain-specific lipid distribution in macrophage plasma membranes. - Domain-specific lipid distribution in macrophage plasma membranes.

Gaus K, Rodriguez M, Ruberu KR, Gelissen I, Sloane TM, Kritharides L, Jessup W. J Lipid Res. 2005 Jul;46(7):1526-38. Epub 2005 May 1.

Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE - Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE

Imai A, Nashida T, Yoshie S, Shimomura H. Arch Oral Biol. 2003 Aug;48(8):597-604.