Anti-SNAP29 antibody [EPR9198(2)]

• Supplier: Abcam
• Catalog: ab181151
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR9198(2)
• Applications: WB ICC/IF ICC/IF FC IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide within Human SNAP29 aa 1-100
• Description: Rabbit Monoclonal
• Gene: SNAP29
• Conjugate: Unconjugated

Product images

All lanes : Anti-SNAP29 antibody [EPR9198(2)] (ab181151) at 1/5000 dilutionLane 1 : Hela cell lysate with NFDM/TBSTLane 2 : 293 cell lysate with NFDM/TBSTLane 3 : Jurkat cell lysate with NFDM/TBSTLysates/proteins at 20 µg per lane.Blocking peptides at 5 % per lane.SecondaryPeroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

Immunofluorescent analysis of -20℃ acetone fixed Hela cells staining SNAP29 using ab181151 at 1/250 dilution and Alexa Fluor®555 stained Goat anti Rabbit IgG at 1/250 as a secondary antibody (red). Dapi counterstain (blue).

Flow cytometric analysis of 2% paraformaldehyde fixed Jurkat cells staining SNAP29 using ab181151 at 1/10 dilution, and FITC conjugated Goat anti rabbit IgG at 1/150 as a secondary antibody (red curve). Rabbit monoclonal IgG was used as the negative control (green curve).

Western blot analysis of SNAP29 in immunoprecipitation pellets from Jurkat cell lysate, using ab181151 at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as a secondary antibody at 1/1000 dilution. Blocking/dilution buffer and concentration: 5% NFDM/TBST