All lanes : Human POLR2F full length protein (ab180291) at 1/20000 dilutionLane 1 : HeLa cell line lysateLane 2 : MCF7 cell line lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-SNX29 antibody [EPR13778] (ab180591) at 1/20000 dilution + Human small intestine tissue lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling SNX29 with ab180591 at 1/100.
Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling SNX29 with ab180591 at 1/100 and dye conjugated secondary Goat anti rabbit IgG at 1/200 (red). Dapi staining is showed in blue.
Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling SNX29 with ab180591 at 1/30 followed by Goat anti rabbit IgG (FITC) at 1/150 (pink). Rabbit monoclonal IgG was used as the Isotype control (green).