All lanes : Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504) at 1/5000 dilutionLane 1 : 293 cell lysateLane 2 : A431 cell lysateLane 3 : Jurkat cell lysateLane 4 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilutiondeveloped using the ECL technique
All lanes : Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504) at 1/1000 dilutionLane 1 : C6 whole cell lysateLane 2 : RAW 264.7 whole cell lysateLane 3 : PC12 whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilutiondeveloped using the ECL technique
Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504) at 1/10000 dilution + Mouse brain lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilutiondeveloped using the ECL technique
Western blot analysis of immunoprecipitation pellet from 1 mg 293 whole cell lysate immunoprecipitated using ab198504 at 1/40 dilution.Lane 1 (Input): 293 whole cell lysate 10µg.Lane 2 (+) : 293 whole cell lysate using ab198504 at 1/1000 dilution.Lane 3 (-) : 293 whole cell lysate using Rabbit monoclonal IgG (ab172730) instead of ab198504.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Western blot analysis of immunoprecipitation pellet from 1 mg PC12 whole cell lysate immunoprecipitated using ab198504 at 1/40 dilution.Lane 1 (Input): PC12 whole cell lysate 10µg.Lane 2 (+) : PC12 whole cell lysate using ab198504 at 1/1000 dilution.Lane 3 (-) : PC12 whole cell lysate using Rabbit monoclonal IgG (ab172730) instead of ab198504.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.