Anti-Somatostatin Receptor 1 antibody
| Name | Anti-Somatostatin Receptor 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2366 |
| Prices | $385.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P IHC-F WB ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide (Rat)A synthetic peptide derived from the C-terminus of rat somatostatin receptor 1 |
| Description | Rabbit Polyclonal |
| Gene | SSTR1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Formalin fixed paraffin embedded human pancreas stained with Somatostatin Receptor using ABC and AEC chromogen.
All lanes : Anti-Somatostatin Receptor 1 antibody (ab2366) at 1 µg/mlLane 1 : PANC-1 (Human pancreatic carcinoma, epithelial-like cell line) Nuclear Lysate (ab14882)Lane 2 : Stomach (Human) Whole Cell Lysate - tumor tissue (ab29678)Lane 3 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab2366 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2366, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.