All lanes : Anti-Sonic Hedgehog antibody (ab114122) at 1 µg/mlLane 1 : E10 Mouse Embryo Brain Tissue LysateLane 2 : Mouse Embryo E10 Tissue LysateLane 3 : E12 Mouse Embryo Brain and Spinal Cord Tissue LysateLane 4 : E14 Mouse Embryo Brain Tissue LysateLane 5 : E14 Ms Embryo Spinal Cord Tissue LysateLane 6 : E16 Ms Embryo Brain Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.