Anti-Sonic Hedgehog antibody [5H4]

• Supplier: Abcam
• Catalog: ab135240
• Prices: $384.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 5H4
• Applications: WB IHC-P FC
• Species Reactivities: Mouse, Human, Monkey
• Antigen: Purified Recombinant fragment, corresponding to amino acids 26-161 of Human Sonic Hedgehog expressed in E
• Description: Mouse Monoclonal
• Gene: SHH
• Conjugate: Unconjugated

Product images

Anti-Sonic Hedgehog antibody [5H4] (ab135240) at 1/500 dilution + Human Sonic Hedgehog recombinant protein

All lanes : Anti-Sonic Hedgehog antibody [5H4] (ab135240) at 1/500 dilutionLane 1 : HEK293 cell lysateLane 2 : HEK293 cell lysate, transfected with Sonic Hedgehog (amino acids 26-161)-hIgGFc

All lanes : Anti-Sonic Hedgehog antibody [5H4] (ab135240) at 1/500 dilutionLane 1 : LNCaP cell lysateLane 2 : HepG2 cell lysateLane 3 : PANC1 cell lysateLane 4 : HeLa cell lysateLane 5 : SK-N-SH cell lysateLane 6 : F9 cell lysateLane 7 : NIH 3T3 cell lysateLane 8 : COS7 cell lysate

Flow cytometric analysis of HeLa cells labelling Sonic Hedgehog with ab135240 at 1/200 dilution (green). Negative control (red).

Immunohistochemical analysis of Paraffin-embedded Human liver cancer tissue labelling Sonic Hedgehog with ab135240 at 1/200 dilution followed by DAB staining.