Anti-Sonic Hedgehog antibody [EP1190Y]
| Name | Anti-Sonic Hedgehog antibody [EP1190Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab53281 |
| Prices | $394.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP1190Y |
| Applications | ICC/IF ICC/IF WB FC IHC-P |
| Species Reactivities | Human |
| Antigen | Synthetic peptide (Human) corresponding to residues within c-product subunit |
| Blocking Peptide | Sonic Hedgehog peptide |
| Description | Rabbit Monoclonal |
| Gene | SHH |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescent staining of HepG2 using anti-Sonic Hedgehog Protein Alexa Fluor 555 RabMAB
![Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281) at 1/2000 dilution + Fetal liver membrane at 10 µgSecondaryGoat anti-rabbit HRP at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/3438_sonic1.png)
Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281) at 1/2000 dilution + Fetal liver membrane at 10 µgSecondaryGoat anti-rabbit HRP at 1/2000 dilution
Human kidney cancerous tissue labelled with ab53281 at 1/100 dilutionHuman kidney cancerous tissue labelled with ab53281 at 1/100 dilution
Immunohistochemical analysis of Human ovarian cancer tissue, staining Sonic Hedgehog with 53281. Antigen retrieval was carried out in citrate buffer using a pressure cooker for 40 minutes. Sections were blocked with blocking agent before incubating with primary antibody (1/2000) for 90 minutes at room temperature. Staining was detected using DAB.
Overlay histogram showing HepG2 cells stained with ab53281 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53281, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Metformin suppresses sonic hedgehog expression in pancreatic cancer cells. - Metformin suppresses sonic hedgehog expression in pancreatic cancer cells.
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Hedgehog related protein expression in breast cancer: gli-2 is associated with - Hedgehog related protein expression in breast cancer: gli-2 is associated with
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FGF10 Signaling differences between type I pleuropulmonary blastoma and - FGF10 Signaling differences between type I pleuropulmonary blastoma and
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Keratocyst of the buccal mucosa: case report and immunohistochemical comparative - Keratocyst of the buccal mucosa: case report and immunohistochemical comparative
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Prognostic value of sonic hedgehog protein expression in gastric cancer. - Prognostic value of sonic hedgehog protein expression in gastric cancer.
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NKT-associated hedgehog and osteopontin drive fibrogenesis in non-alcoholic fatty - NKT-associated hedgehog and osteopontin drive fibrogenesis in non-alcoholic fatty
Syn WK, Agboola KM, Swiderska M, Michelotti GA, Liaskou E, Pang H, Xie G, Philips G, Chan IS, Karaca GF, Pereira Tde A, Chen Y, Mi Z, Kuo PC, Choi SS, Guy CD, Abdelmalek MF, Diehl AM. Gut. 2012 Sep;61(9):1323-9.
Inhibition of the sonic hedgehog pathway by cyplopamine reduces the CD133+/CD15+ - Inhibition of the sonic hedgehog pathway by cyplopamine reduces the CD133+/CD15+
Schiapparelli P, Shahi MH, Enguita-German M, Johnsen JI, Kogner P, Lazcoz P, Castresana JS. Cancer Lett. 2011 Nov 28;310(2):222-31.
Inhibition of Hedgehog signaling antagonizes serous ovarian cancer growth in a - Inhibition of Hedgehog signaling antagonizes serous ovarian cancer growth in a
McCann CK, Growdon WB, Kulkarni-Datar K, Curley MD, Friel AM, Proctor JL, Sheikh H, Deyneko I, Ferguson JA, Vathipadiekal V, Birrer MJ, Borger DR, Mohapatra G, Zukerberg LR, Foster R, Macdougall JR, Rueda BR. PLoS One. 2011;6(11):e28077.