All lanes : Anti-SORBS1 antibody (ab4551)Lane 1 : Molecular weight marker (Bio-Rad Precision Plus Kaleidoscope standards)Lane 2 : 10 µl Human plasma 1 (600-800 µg protein)Lane 3 : 10 µl Human plasma 2 (600-800 µg protein)Lane 4 : 10 µl Human White Blood cells 1 (30-50 µg protein)Lane 5 : 10 µl Human White Blood cells 2 (30-50 µg protein)Lane 6 : 10 µl HEK293 whole cell lysate (prepared by scrapping 10 cm confluent dish into 4ml of 2x sample buffer (Sigma)SecondaryDonkey anti-goat IgG (H+L) conjugated to Alexa-Fluor680 at 1/5000 dilution
ab4551 (2.5 µg/ml) staining SORBS1 in Human Skeletal Muscle by IHC-P. Steamed antigen retrieval in citrate buffer pH6, AP-staining. This image shows spotty staining on muscle fibres in longitudinal section.