ab94396 staining SOX5 in mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 3% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/500) for 12 hours at 4°C. An Alexa Flour® 555-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview
ab94396 staining SOX5 in mouse cartilage tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 1% Triton in PBS and blocked with 10% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 10% serum) for 16 hours at 4°C. An Alexa Flour® 488-conjugated goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.See Abreview
Anti-SOX5 antibody - ChIP Grade (ab94396) at 1 µg/ml + Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.