All lanes : Anti-SP1 (phospho T453) antibody (ab37707) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SP1 (phospho T453) peptide (ab38350) at 1 µg/mlLane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human SP1 (phospho T453) peptide (ab38350) at 1 µg/mlLane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SP1 peptide (ab42654) at 1 µg/mlLane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human SP1 peptide (ab42654) at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab37707, at a 1/2000 dilution, staining human SP1 (phospho T453) in colon cancer tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.See Abreview
All lanes : Anti-SP1 (phospho T453) antibody (ab37707) at 1/500 dilutionLane 1 : Nuclear lysate prepared from rat intestinal epithelial cellsLane 2 : Nuclear lysate prepared from Caco2 cellsLysates/proteins at 50 µg per lane.SecondaryGoat anti-rabbit polyclonal conjugated to HRPdeveloped using the ECL technique