Anti-SP1 (phospho T453) antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab59257
• Prices: $403.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ELISA IHC-P WB ChIP ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic phosphopeptide derived from human SP1 around the phosphorylation site of threonine 453 (I-R-TP-P-T)
• Description: Rabbit Polyclonal
• Gene: SP1
• Conjugate: Unconjugated

Product images

Immunofluorescence analysis of HeLa cells, using ab59257 Antibody. The picture on the right is treated with the synthesized peptide.

Wild type MEF cells (2 × 107 cells) were cross-linked with formaldehyde, quenched with glycine, resuspended in SDS lysis buffer (1% SDS, 10 mm EDTA, 50 mm Tris-HCl, pH 8.0, with protease inhibitors and phosphatase inhibitors), sonicated on ice, and centrifuged at 4 °C. Supernatant (400 μl) were diluted to a final volume of 4 ml in a mixture of 9 parts dilution buffer (1% Triton X-100, 150 mm NaCl, 2 mm EDTA, 20 mm Tris-HCl, with protease inhibitors, pH 8.0) and 1 part lysis buffer.Mixtures were incubated with 4 μg of anti-p-Sp1 (ab 59257) or anti-Sp1 antibodies with rotating at 4 °C overnight followed with incubation with 100 μl of protein A beads with rotating at 4 °C for 4 h. After gentle centrifugation (2000 rpm), beads were resuspended in 1 ml of wash buffer (1% Triton X-100, 0.1% SDS, 150 mm NaCl, 2 mm EDTA, 20 mm Tris-HCl, with protease inhibitors, pH 8.0) and washed with wash buffer 3 times followed by one wash with a final wash buffer (1% Triton X-100, 0.1% SDS, 500 mm NaCl, 2

All lanes : Anti-SP1 (phospho T453) antibody - ChIP Grade (ab59257) at 1/500 dilutionLane 1 : A549 cell extractsLane 2 : A549 cell extracts with immunising phospho peptide

ab59257, at 1/50 dilution, staining SP1 in paraffin embedded human brain tissue by Immunohistochemistry in the absence or presence of the immunising peptide.

ab59257 (1:1000) Antibody detects endogenous levels of SP1 only when phosphorylated at Thr453.

Product References

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Cailotto F, Reboul P, Sebillaud S, Netter P, Jouzeau JY, Bianchi A. J Biol Chem. 2011 Jun 3;286(22):19215-28.

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