All lanes : Anti-Sp7 / Osterix antibody [EP11344(2)] (ab187158) at 1/1000 dilutionLane 1 : Saos2 cell lysateLane 2 : SW1353 cell lysateLane 3 : Jurkat cell lysateLane 4 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Sp7 / Osterix antibody [EP11344(2)] (ab187158) at 1/1000 dilutionLane 1 : Raw264.7 cell lysateLane 2 : PC-12 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow Cytometry analysis of Jurkat cells labeling Sp7 / Osterix using ab187158 at a 1/260 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.