Anti-Sp7 / Osterix antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab22552
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-F ChIP ICC/IF ICC/IF WB IHC-P IP IHC-F
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse Sp7/ Osterix
• Description: Rabbit Polyclonal
• Gene: SP7
• Conjugate: Unconjugated

Product images

ab22552 staining Sp7/ Osterix in C2C12 cells by Immunocytochemistry/ Immunofluorescence.Cells were stained with ab22552 at a 1/500 dilution followed by goat anti-rabbit IgG conjugated with Alexa Fluor 488 at a 1/400 dilution. Labeling was detected using a Leica TCS SL inverted laser scanning confocal microscope.The subcellular localization of Osterx in C2C12 cells was analyzed by immunofluorescence with ab22552 in cells either transiently transfected with short Osterix construct or incubated in medium without serum. Scale bar 10 µm.

The image shows Osterix staining in rat bone tibiae using ab22552 at 1/100. See Abreview

Image courtesy of Human Protein Atlasab22552 staining Sp7 / osterix in human bone marrow tissue. Paraffin embedded human bone marrow tissue was incubated with ab22552 (1/25 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.ab22552 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.

IHC image of Sp7/Osterix staining in human tonsil FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22552, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

ab22552 staining Sp7 / Osterix in 21 days old rat developing long bone tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with methanol and blocking with 20% serum at 220C for 30 minutes was performed. The sample was incubated with primary antibody (1/100) for 3 hours at 220C. A Cy3®-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/1000 dilution.See Abreview

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