All lanes : Anti-SPAG7 antibody [EPR13391-33] (ab181184) at 1/2000 dilutionLane 1 : MCF7 cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunoprecipitation. ab181184 at 1/1000 labeling SPAG7 immunoprecipitated from HeLa cell lysate using ab181184 at 1/60.Lane 2: Control IgG.
Immunofluorescent analysis of MCF7 cells labeling SPAG7 with ab181184 at 1/250 with Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 and DAPI staining (blue).