All lanes : Anti-SPANX antibody (ab51493) at 1/1000 dilutionLane 1 : VWM105 cell nuclear extract Lane 2 : purified recombinant SPANX-C fusion proteinLane 3 : purified recombinant SPANX-BLane 4 : purified recombinant SPANX-CLane 5 : purified recombinant SPANX-NSecondaryLane 1 : IRDye™800 conjugated Goat anti Rabbit IgG [H&L] Lane 2 : IRDye™800 conjugated Goat anti Rabbit IgG [H&L]
ab51493 at 2.5 ug/ml staining human sperm and spermatids at 60X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.