Immunofluorescent staining of Human cell line U-2 OS shows positivity in nuclei but not nucleoli. Recommended concentration of ab121706 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121706, at 1/450 dilution, staining SPATA20 in paraffin-embedded Human testis tissue by Immunohistochemistry.
All lanes : Anti-SPATA20 antibody (ab121706) at 1/250 dilutionLane 1 : RT-4 cell lysateLane 2 : U-251 MG cell lysateLane 3 : Human Plasma lysateLane 4 : Human Liver lysateLane 5 : Human Tonsil Lysate
Immunohistochemical staining of Human testis shows general distinct nuclear positivity which in some cells of the seminiferus ducts was combined with cytoplasmic staining using ab121706