All lanes : Anti-SPATA5 antibody [EPR14038] (ab189519) at 1/10000 dilutionLane 1 : 293 cell lysateLane 2 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
All lanes : Anti-SPATA5 antibody [EPR14038] (ab189519) at 1/10000 dilutionLane 1 : A431 cell lysateLane 2 : Human testis lysateLysates/proteins at 20 µg per lane.SecondaryGoat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling SPATA5 with ab189519 at 1/80 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti-rabbit IgG (FITC) secondary antibody at 1/150 dilution.