Anti-SPC24 antibody [EPR11548(B)]

• Supplier: Abcam
• Catalog: ab169786
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR11548(B)
• Applications: WB IP ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Synthetic peptide, corresponding to residues in Human SPC24 (UniProt ID: Q8NBT2)
• Description: Rabbit Monoclonal
• Gene: SPC24
• Conjugate: Unconjugated

Product images

ab169786 staining SPC24 in the HeLa cell line from Human cervical cancer by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody (1/200 in PBS) for 16 hours at 22°C. ab150081 an Alexa Fluor®488-conjugated Goat anti-rabbit IgG H&L polyclonal(1/200) was used as the secondary antibody. Nuclei were stained with DAPISee Abreview

All lanes : Anti-SPC24 antibody [EPR11548(B)] (ab169786) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : MOLT4 cell lysateLane 4 : TF1 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique

Detection of SPC24 by Western Blot of Immunprecipitate. 293 cell lysate (1) or 1X PBS (2) immunoprecipitated using ab169786 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.