Anti-SPHK1 antibody
| Name | Anti-SPHK1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab37980 |
| Prices | $379.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | KLH conjugated synthetic peptide selected from the sequence of human SPHK1 within aa 1-100 |
| Description | Rabbit Polyclonal |
| Gene | SPHK1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab37980 stained HepG2 cells. The cells were 4% Formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37980, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Lane 1 : c-myc tagged SPHK1 antibodyLane 2 : Anti-SPHK1 antibody (ab37980) at 0.25 mg/mlLane 1 : transfected 293 cell lysateLane 2 : transfected 293 cell lysate