Anti-SPON1 antibody
| Name | Anti-SPON1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab40797 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Bovine, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Rat SPON1 |
| Blocking Peptide | Human SPON1 peptide |
| Description | Rabbit Polyclonal |
| Gene | SPON1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC-FoFr image of SPON1 staining on Mouse Spinal Cord using ab40797 (1:1000).The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the spinal cord were cryoprotected in sucrose 30% overnight. Spinal cords were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.See Abreview
All lanes : Anti-SPON1 antibody (ab40797) at 2 µg/mlLane 1 : Brain (Rat) Tissue LysateLane 2 : Spinal Cord (Rat) Tissue Lysate Lane 3 : Ovary (Mouse) Tissue Lysate Lane 4 : Mouse (14 day old) Fetal Tissue Lysate Lane 5 : Brain (Rat) Tissue Lysate with Human SPON1 peptide (ab41539) at 1 µg/mlLane 6 : Spinal Cord (Rat) Tissue Lysate with Human SPON1 peptide (ab41539) at 1 µg/mlLane 7 : Ovary (Mouse) Tissue Lysate with Human SPON1 peptide (ab41539) at 1 µg/mlLane 8 : Mouse (14 day old) Fetal Tissue Lysate with Human SPON1 peptide (ab41539) at 1 µg/mlLysates/proteins at 15 µg per lane.developed using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab40797 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40797, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
F-spondin deficient mice have a high bone mass phenotype. - F-spondin deficient mice have a high bone mass phenotype.
Palmer GD, Attur MG, Yang Q, Liu J, Moon P, Beier F, Abramson SB. PLoS One. 2014 May 29;9(5):e98388.
F-spondin gene transfer improves memory performance and reduces amyloid-beta - F-spondin gene transfer improves memory performance and reduces amyloid-beta
Hafez DM, Huang JY, Richardson JC, Masliah E, Peterson DA, Marr RA. Neuroscience. 2012 Oct 25;223:465-72.