Anti-SREBP2 antibody
| Name | Anti-SREBP2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab30682 |
| Prices | $394.00 |
| Sizes | 250 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F WB ICC/IF |
| Species Reactivities | Mouse, Rat, Chicken, Human, Guinea Pig, Pig, Xenopus, Hamster |
| Antigen | Synthetic peptide: SPLLDDAKVKDEPDS , corresponding to amino acids 455 - 469 of Human SREBP2 Run BLAST with Run BLAST with |
| Blocking Peptide | SREBP2 peptide |
| Description | Rabbit Polyclonal |
| Gene | SREBF2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-SREBP2 antibody (ab30682) at 2.5 µg/mlLane 1 : Human fibroblast cell lysateLane 2 : Rat brown fat homogenateLane 3 : Rat testis supernatantLysates/proteins at 60 µg per lane.
ICC/IF image of ab30682 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30682, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Overactivation of intestinal SREBP2 in mice increases serum cholesterol. - Overactivation of intestinal SREBP2 in mice increases serum cholesterol.
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Identification of miR-185 as a regulator of de novo cholesterol biosynthesis and - Identification of miR-185 as a regulator of de novo cholesterol biosynthesis and
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Alpha-lipoic acid reduces LDL-particle number and PCSK9 concentrations in - Alpha-lipoic acid reduces LDL-particle number and PCSK9 concentrations in
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Sterol regulatory element-binding proteins are regulators of the rat thyroid - Sterol regulatory element-binding proteins are regulators of the rat thyroid
Rauer C, Ringseis R, Rothe S, Wen G, Eder K. PLoS One. 2014 Mar 13;9(3):e91265.
A novel processing system of sterol regulatory element-binding protein-1c - A novel processing system of sterol regulatory element-binding protein-1c
Nakakuki M, Kawano H, Notsu T, Imada K, Mizuguchi K, Shimano H. J Biochem. 2014 May;155(5):301-13.
Protein phosphatase 2A (PP2A) regulates low density lipoprotein uptake through - Protein phosphatase 2A (PP2A) regulates low density lipoprotein uptake through
Rice LM, Donigan M, Yang M, Liu W, Pandya D, Joseph BK, Sodi V, Gearhart TL, Yip J, Bouchard M, Nickels JT Jr. J Biol Chem. 2014 Jun 13;289(24):17268-79.
The epigenetic drug 5-azacytidine interferes with cholesterol and lipid - The epigenetic drug 5-azacytidine interferes with cholesterol and lipid
Poirier S, Samami S, Mamarbachi M, Demers A, Chang TY, Vance DE, Hatch GM, Mayer G. J Biol Chem. 2014 Jul 4;289(27):18736-51.
Endoplasmic reticulum stress mediates amyloid beta neurotoxicity via - Endoplasmic reticulum stress mediates amyloid beta neurotoxicity via
Barbero-Camps E, Fernandez A, Baulies A, Martinez L, Fernandez-Checa JC, Colell A. Am J Pathol. 2014 Jul;184(7):2066-81.
Combination of intrauterine growth restriction and a high-fat diet impairs - Combination of intrauterine growth restriction and a high-fat diet impairs
Zinkhan EK, Chin JR, Zalla JM, Yu B, Numpang B, Yu X, Jiang C, Callaway CW, McKnight RA, Joss-Moore L, Lane RH. Pediatr Res. 2014 Nov;76(5):432-40.
Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function - Disruption of astrocyte-neuron cholesterol cross talk affects neuronal function
Valenza M, Marullo M, Di Paolo E, Cesana E, Zuccato C, Biella G, Cattaneo E. Cell Death Differ. 2015 Apr;22(4):690-702.