All lanes : Anti-SRP72 antibody [EPR17143] (ab200199) at 1/5000 dilutionLane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) cell lysateLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-SRP72 antibody [EPR17143] (ab200199) at 1/5000 dilution + Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-SRP72 antibody [EPR17143] (ab200199) at 1/1000 dilution + Human fetal brain lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling SRP72 with ab200199 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.