Immunofluorescent analysis of Neuro-2a cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling SRPK2 with ab192238 at 1/250 dilution (5.4 μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).Negative controls: anti-SRPK2 at 1/250 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling SRPK2 with ab192238 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SRPK2 with ab192238 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
All lanes : Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238) at 1/1000 dilution + Human fetal kidney lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238) at 1/2000 dilution + Human fetal brain lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution